3D4/21 豬肺泡巨噬細胞

簡要描述：3D4/21 豬肺泡巨噬細胞，ATCC 細胞|細胞系|細胞株|菌種；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件！

產品型號：
廠商性質：生產廠家
更新時間：2025-09-11
訪問量：3603

產品咨詢聯系我們

產品分類

Product Category

細胞庫

細胞原代牛、狗、雞、鴨、魚原代細胞羊原代細胞豬原代細胞兔原代細胞小鼠原代細胞大鼠原代細胞人原代細胞培養基原代細胞凍存液 ATCC 人乳腺癌豬正常細胞豬睪丸細胞系人膠質瘤細胞人成骨細胞人永生化肝細胞人肝癌細胞人卵巢癌細胞人結直腸腺癌人經典小細胞肺癌細胞人小細胞肺癌細胞人結直腸腺癌細胞人鼻腔上皮細胞中國倉鼠卵巢懸浮細胞嗜性逆轉錄病毒包裝穩定細胞株小鼠神經細胞瘤人眼脈洛膜黑色素瘤 PT67 逆轉錄酶病毒包裝細胞鴨子胚胎成纖維細胞豬腎細胞系昆蟲細胞貓腎細胞恒河猴胚腎細胞倉鼠卵巢細胞負鼠腎細胞倉鼠肺細胞果蠅胚胎細胞家貓肺成纖維樣細胞雞胚細胞豚鼠心肌來源細胞豚鼠肺成纖維樣細胞豚鼠皮膚成纖維樣細胞狗腎細胞非洲綠猴胚胎腎上皮細胞猴胚胎腎上皮細胞長臂猿淋巴瘤新生牛眼囊成纖維細胞黑化大家鼠肺成纖維樣細胞俄勒岡地鼠細胞家兔皮膚成纖維樣細胞昆蟲卵巢細胞人胸膜瘤細胞非洲綠猴腎細胞 VERO 衍生株（SVP）白化金黃地鼠肺成纖維樣細胞黃胸鼠肺成纖維樣細胞白鰱尾鰭細胞蝙蝠肺細胞草魚腎細胞系大黃魚腎細胞猴腎細胞系犬腎細胞系人結腸直腸腺癌豬肺泡巨噬細胞 PFHSA05（TLL2）人重組特洛德樣蛋白因子 PFHSA04 （BMP1）人重組骨誘導因子人乳腺癌細胞結腸癌細胞成纖維細胞人胚腎細胞動物卵巢細胞動物胚胎細胞動物肺細胞肋骨來源細胞成纖維樣細胞動物上皮細胞動物肝臟細胞可誘導表達穩定細胞株動物氣管細胞動物腎細胞動物巨噬細胞人正常細胞人腫瘤細胞、癌細胞小鼠正常細胞小鼠腫瘤細胞、癌細胞大鼠正常細胞大鼠腫瘤細胞其它動物細胞查看全部產品

詳細介紹

3D4/21 豬肺泡巨噬細胞
3D4/21 (ATCC
^® CRL-2843

Organism	Sus scrofa, pig
Tissue	lung
Cell Type	macrophage macrophage (alveolar); immortalized with SV40 large T antigen transformed with pSV3-neo
Product Format	frozen
Morphology	macrophage
Culture Properties	adherent
Biosafety Level	2 [Cells contain SV40 viral DNA sequences]
Age	27 days
Gender	unknown
Strain	Landrace
Applications	These porcine myelomonocytic cell lines may have a wide variety of applications in porcine virology and immunology Ref. 3D4/21 豬肺泡巨噬細胞
Storage Conditions	liquid nitrogen vapor phase

Derivation	The parental porcine monomyeloid cell line, 3D4, was established in December of 1998 following transfection of primary porcine alveolar macrophage cultures with the pSV3neo plasmid. Single cell cloning and selection in G-418 of the 3D4 parental cell line resulted in establishment of 3D4/2 ( ATCC CRL-2845), 3D4/21 (ATCC CRL-2843) and 3D4/31 (ATCC CRL-2844).
Virus Susceptibility	Bovine adenovirus 3 Classical swine fever virus , Classical swine fever virus Human parainfluenza virus 3 Swinepox virus Vesicular stomatitis New Jersey virus Porcine adenovirus Herpes simplex virus 1 African swine fever virus Pseudorabies virus Vaccinia virus Swine vesicular disease virus
Comments	The plasmid carries the genes for neomycin resistance and SV40 large T antigen. A subpopulation of each cell line (3D4/2 (ATCC CRL-2845), 3D4/21 (ATCC CRL-2843) and 3D4/31 (ATCC CRL-2844)) was positive, to varying degrees depending on the media formulation, for nonspecific esterase activity and phagocytosis. Clone 3D4/21 can produce Bovine adenovirus type 3 (BAV-3) to markedly higher titers than clones 3D4/2 and 3D4/31. Addition of DMSO improved the capability of clone 3D4/21 to replicate the field isolate of African swine fever virus (ASFV/Lillie) compared to the other clones.

Derivation

The parental porcine monomyeloid cell line, 3D4, was established in December of 1998 following transfection of primary porcine alveolar macrophage cultures with the pSV3neo plasmid.

Single cell cloning and selection in G-418 of the 3D4 parental cell line resulted in establishment of 3D4/2 (

ATCC CRL-2845), 3D4/21 (ATCC CRL-2843) and 3D4/31 (ATCC CRL-2844).

Virus Susceptibility

Bovine adenovirus 3

Classical swine fever virus , Classical swine fever virus

Human parainfluenza virus 3

Swinepox virus

Vesicular stomatitis New Jersey virus

Porcine adenovirus

Herpes simplex virus 1

African swine fever virus

Pseudorabies virus

Vaccinia virus

Swine vesicular disease virus

Comments

The plasmid carries the genes for neomycin resistance and SV40 large T antigen.

A subpopulation of each cell line (3D4/2 (ATCC CRL-2845), 3D4/21 (ATCC CRL-2843) and 3D4/31 (ATCC CRL-2844)) was positive, to varying degrees depending on the media formulation, for nonspecific esterase activity and phagocytosis.

Clone 3D4/21 can produce Bovine adenovirus type 3 (BAV-3) to markedly higher titers than clones 3D4/2 and 3D4/31.

Addition of DMSO improved the capability of clone 3D4/21 to replicate the field isolate of African swine fever virus (ASFV/Lillie) compared to the other clones.

Complete Growth Medium	RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10%

Subculturing	Volumes used in this protocol are for 75cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 5 x 10³ to 7 x 10³ viable cells/cm² is recommended. Incubate cultures at 37°C. Subculture when cell concentration reaches between 3 x 10⁵ and 4 x 10⁵cells/cm². *Subction Ratio:** A subction ratio of 1:6 to 1:8 is recommended Medium Renewal:* Two to three times weekly Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.
Cryopreservation	Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: 5% CO₂ in air recommended

Subculturing

Volumes used in this protocol are for 75cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 5 x 10³ to 7 x 10³ viable cells/cm² is recommended.
Incubate cultures at 37°C. Subculture when cell concentration reaches between 3 x 10⁵ and 4 x 10⁵cells/cm².

Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:8 is recommended

Medium Renewal: Two to three times weekly

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.

Cryopreservation

Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.

Culture Conditions

Temperature: 37°C

Atmosphere: 5% CO₂ in air recommended

產品咨詢

上一篇：ATCC 700324 產酸克雷伯菌
下一篇：CRL-28433D4/21 豬肺泡巨噬細胞

3D4/21 豬肺泡巨噬細胞

3D4/21 豬肺泡巨噬細胞
3D4/21 (ATCC
^® CRL-2843

3D4/21 豬肺泡巨噬細胞

留言框

產品：

您的單位：

您的姓名：

聯系電話：

常用郵箱：

省份：

詳細地址：

補充說明：

驗證碼：

3D4/21 豬肺泡巨噬細胞

3D4/21 豬肺泡巨噬細胞3D4/21 (ATCC® CRL-2843

3D4/21 豬肺泡巨噬細胞

留言框

產品：

您的單位：

您的姓名：

聯系電話：

常用郵箱：

省份：

詳細地址：

補充說明：

驗證碼：

3D4/21 豬肺泡巨噬細胞
3D4/21 (ATCC
^® CRL-2843